Tokai Hit Co., Ltd.

 Stage Top Incubator STX series add-on option

Perfusion/Media exchange without removing a dish lid is possible.

Prevents media evaporation and contamination during long-term imaging.

System Image

Specifications

 Stage Top Incubator STX series add-on option

Rapid and vibration-free drug delivery is possible.

Prevents media evaporation and contamination during long-term imaging.

System Image

Specifications

 Stage Top Incubator STX series add-on option

Creates hypoxia and normal conditions with easy settings.

Specifications

 Stage Top Incubator add-on option

 By adding an external humidifier to the Tokai Hit incubation system,
it is possible to perform long-term culture for 3 to 4 days without refilling the water to the chamber during imaging.

System Image

仕様

 Stage Top Incubator add-on option

 Perfusion, Media Exchange, Drug Delivery and Mixing can be easily programmed and done without disturbing your sample.

You can use the commercially available 35 mm dish.

System Image

Specifications

Pump unit can be installed in a CO₂  incubator

【Components】

・Controller, Pump, Tube set

Inside the incubator

【Features】

Pump unit can be installed in a CO₂ incubator

Adopts moisture-proof design and shield technology.
It is possible to install a perfusion system including a pump in a CO₂ incubator.

Programmatic operation of continuous perfusion and media exchange is possible

Any protocol can be created by combining continuous perfusion and media exchange.
(Maximum 8 steps, 14 repeats)

Reliable perfusion with leveling function

Reproducible media exchange is possible with our unique leveling technology.
Maintains a stable amount of media during long-term continuous perfusion and high-flow media exchange.

【Basic specifications】

【Application】

・Media Exchange
・Perfusion Culture
・Tissue Engineering
・3D Culture
・Organoid
・Biomimetics

Sealed lids and Dish attachment for 35mm dish

Sealed lids with pipes for perfusion and drug delivery
Dish attachment for 35mm dish x 2 pcs

Compatible with：Corning / Matsunami / MatTek / Eppendorf / Nunc / Greiner

【Image】

 【Features】

Non-contact pressure measurement

It measures the flow path pressure during perfusion aseptically.
By feeding back to the pump, constant pressure liquid feeding is also possible.

Pump unit can be installed in a CO₂ incubator

Adopts moisture-proof design and shield technology
It is possible to install a perfusion system including a pump in a CO₂ incubator.
It controls the two channels independently.

Touch panel controller

You can intuitively switch between constant pressure/pulsation constant pressure/constant flow rate perfusion mode.
The data logging function is available as standard.

【Components】

	Controller It controls the pump and set the parameter. A touch panel is used to operate the pump at a pulsating constant pressure, constant pressure, and constant flow rate.
	Pump The pumps are in a container with a moisture-proof design and shield technology. It controls each of the two-channel lines and the two pumps independently. There is one pump that can be used for general purposes such as drainage in the container.
	Buffer bottle Using a unique mechanism that measures the flow path pressure via a buffer bottle. Aseptic and reusable sensing of channel pressure is possible.

【Specifications】

Offers precision temperature, humidity and CO₂ control for cell culture on a microscope.
Enables to conduct short and long term (more than 2 weeks) Time-Lapse Imaging.
 

By applying Real-time Sample Feedback Regulation, it can maintain an optimal cell culture environment
which is as same as the conventional CO₂ incubator.
 

With our variety of options, we can offer solutions for abundant applications, and leading the experiment to success.

【Features】

Accurate and uniform temperature control

	TOKAI HIT Heating Quality Tokai Hit's original Top Heater is proven to distribute heat uniformly within the Chamber regardless of the type of vessels.
	No interference by objective With unique Top Heater Heating Time regulation, the bottom of Chamber is access-free for variety of objectives. (No metal plate at the bottom.)
	Real-time Sample Feedback Regulation Sterilized temperature sensor and magnetic lids make it easy to measure the temp. of culture media upon research needs. The controller regulates the heater based on the sensor signal to keep sample at the target temp. accurately.

Apply Internal Humidifier to keep high humidity condition during the imaging

Keeps high humidity inside the chamber by heating the distilled water in the Bath Unit. Internal Humidifier minimizes the change in concentration of media by keeping the humidity inside the chamber.

Stable CO₂ environment

The controller mixes 100%CO2 gas and the surrounding air automatically. Stable gas concentration inside the Chamber is obtained by keep sending the mixed gas to the Chamber. (※In case of controller with a built-in digital gas mixer)

【Line-up】

We offer various lineups depending on the microscope, stage, and vessels etc.

Please feel free to contact us anytime.

Suitable for sterility operation during imaging

Enable to achieve clean bench cleanness/sterility environment on the microscope.

Recommend when:

• CARE the cleanness of microscope environment during operation.
• Image cells after clean bench manipulation (e.g. induce cell differentiation by media exchange, etc)
• For temperature sensitive sample imaging.
• Wish to remove vessels lid for conducting drug delivery/media exchange during time-lapse imaging.
• Transplant the imaged cells/sample.
• For the sample which cannot add antibiotics into the media. The real live cell imaging without antibiotics possible.

Target applications:

• iPS cells/researches
• Organoid study
• Pharmceutical field
• Food research
• Embryo study, etc.

Same sterility level as the clean bench

Achieve same sterility as level 100 (ISO class 5) on the microscope and maintain cleanliness levels during imaging.

Compatible with complex microscope applications

Installation of microscope add-on system such as confocal equipment is possible
that clean bench cannot to accommodate.

Red LED light and contactless LED switch

Red LED (longer wavelength) is integrated. White and red lights can be switched with a single switch. With the contactless switch, you can turn on/off the LED light without touching the system during experiments.

【Line-up】

Applicable microscope	Model	Image
Olympus IX83	IX83PBS
Nikon Ti2	TI2PBS
Leica DMi8	DMi8PBS

Depending on the accessories (camera, stage, etc.), the model may be a customized model. Please contact us for details.

Suitable for microscope environemt control.

It allows to prevent focus drift caused by environmental disturbances and keep the preheat-media (bottle), objectives, etc under same temperature.

No duct required

Saves your working and setting space with built-in fan heaters. No air-duct is required for heating.

Anti-vibration heater

With anti-vibration design, the system can be used under confocal without image drift.

Simple Dark Room use

The Dark type can prevent sample from light exposure and work as simple dark room.

【Line-up】

Depending on the accessories (camera, stage, etc.), the model may be a customized model. Please contact us for details.